In Vitro fertilisation is the means of fertilising eggs away from body. It is commonly attempted in cases of infertility. In this technique, the eggs from a woman are taken by way of a needle and incubated with sperms from her partner or perhaps a donor. This results in fertilisation and the fertilised egg is transferred back to the uterus to grow.
What are the necessary requirements for IVF?
IVF requires three basic necessities, a wholesome egg, a wholesome sperm and healthy uterus that may carry the pregnancy. This question is necessary because it's not necessary to have all three to make a bay. Let's like consider women who are unable to produce eggs, but has a functional uterus. In cases like this egg donation is a reasonable option. Similarly if sperms of the male partner are defective then donor sperms can also be used. This really is called surrogacy. There's another form of surrogacy; many women end up having their uterus which prevents them from carrying a pregnancy. The embryos produced by fertilisation of their eggs using their partner's sperms may be transferred to a different woman who has functioning uterus. hcg
Are you able to describe the process?
You'll first get a class of hormonal drugs to improve your ovaries to create more follicles (follicles contain eggs). These hormones are mostly FSH analogues and they stimulate the ovaries to create between 50-60 such follicles. The growth of those follicles will undoubtedly be monitored closely by testing for hormones and serial ultrasound scans. On across the 10th day a go of inject able HCG is given which in turn causes these follicles to mature further. These follicles are then aspirated by a needle passed through the vagina and around 30 of those follicles will undoubtedly be aspirated at one time. It will require about 20 min to complete this procedure and during this time women will either be under sedation or general anaesthesia. hcg shots
Once these follicles are aspirated, they are sent to the lab where they'll be processed. The eggs are cleaned of their surrounding cells and the very best eggs for fertilisation are selected. They then mixed with washed sperms (sperms which are removed of inactive cells and seminal fluid) in a petridish and incubated at 37 degree Celsius for 18 hours. During this time period the fertilised egg will have two pronuclei.In cases when the sperm has difficulty penetrating the ovum, it could be directly injected into the egg by a procedure called Intra-cytoplasmic Sperm Injection.Once the eggs are fertilised they are used in a culture chamber where they grow for the next 3-5 days before being implanted in the female. These embryos might be cultured in artificial culture media or endometrial co-culture. In endometrial coculture the embryo is cultured on cells from woman's own endometrium. hcg
Anytime 3-5 embryos are positioned in the uterus for implantation dependant on many factors.
This was a brief summary concerning the procedure. You can read the whole article in In Vitro Fertilisation such as the success rates, complications and other conditions associated with this particular procedure from my website.